Composite

Part:BBa_K1725428:Design

Designed by: Andrey Filipov   Group: iGEM15_Glasgow   (2015-09-08)


J23114 + RBS + UirR + B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 510


Design Notes

The regulator and J23114 were ligated to B0015 after restriction that was followed by an Azure-A-based gel extraction.


Source

The regulator comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.

References